The direct colorimetric estimation of reducing sugars and other reducing substances with tetrazolium salts
نویسندگان
چکیده
منابع مشابه
The direct colorimetric estimation of reducing sugars and other reducing substances with tetrazolium salts.
Since Kuhn & Jerchel (1941) showed that tetrazolium salts were reduced by bacteria, yeasts and germinating seeds, 2:3:5-triphenyltetrazolium chloride or bromide has come into prominence as a viability testing reagent (Cottrell, 1947) and for vital staining (Mattson, Jensen & Dutcher, 1947). Its specific advantages for this purpose and for the purposes which follow lie in its Eo value of -008 V....
متن کاملSemi-micro Estimation of Reducing Sugars.
The estimation of sugars is a matter of interest to every worker engaged in the study of carbohydrate metabolism and related problems. In order to be of any real value a method must not only allow the determination of relatively small amounts of sugars with sufficient accuracy, but should also be rapid, and thus permit of a large number of determinations being carried out in a short time. Semi-...
متن کاملThe Determination of Reducing Sugars
In the course of an investigation, still in progress, of the physiological activity of a group of soil organisms, it became necessary to determine the quantity of dextrose in a series of solutions. It was desirable to have a method as accurate as the Fehling gravimetric one but which would yield results about as quickly as by direct titration. The reducing sugar present in the 150 cc. of each o...
متن کاملColorimetric Method for Determination of Sugars and Related Substances
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars...
متن کاملThe Role of Different Sugars, Amino Acids and Few Other Substances in Chemotaxis Directed Motility of Helicobacter Pylori
Objective(s) Motility plays a major role in pathogenicity of Helicobacter pylori, yet there is scarce data regarding its chemotactic behaviour. The present study was designed to investigate the chemotactic responses of local isolates of H. pylori towards various sugars, amino acids, as well as some other chemical substances. Materials and Methods Chemotaxis was assayed by a modified Adler’s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1951
ISSN: 0306-3283
DOI: 10.1042/bj0490423